CDCP1 Protein (AA 29-667) (His-Avi Tag,Biotin)

Catalog No. ABIN7274292

Product Details

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Protein Type: Recombinant

Protein Characteristics: AA 29-667

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This CDCP1 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Cynomolgus CDCP1 Protein

Sequence: Ala29-Thr667

Characteristics: Recombinant Biotinylated Cynomolgus CDCP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala29-Thr667.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CDCP1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CDCP1, His Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Alternative Name: CDCP-1 (CDCP1 Products)

Synonyms: CD318 Protein, SIMA135 Protein, TRASK Protein, 9030022E12Rik Protein, AA409659 Protein, E030027H19Rik Protein, RGD1305578 Protein, zgc:113071 Protein, CUB domain containing protein 1 Protein, CUB domain containing protein 1a Protein, CDCP1 Protein, Cdcp1 Protein, cdcp1a Protein

Background: Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Molecular Weight: 75 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_001114659