P-Cadherin Protein (CDH3) (AA 108-654) (His tag)

Catalog No. ABIN7274304

This Recombinant P-Cadherin protein is produced in HEK-293 Cells.

Quick Overview for P-Cadherin Protein (CDH3) (AA 108-654) (His tag) (ABIN7274304)

Target: P-Cadherin (CDH3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 108-654

Purification tag / Conjugate: This P-Cadherin protein is labelled with His tag.

Purpose: Human CDH3/Cadherin 3 Protein

Sequence: Asp108-Gly654

Characteristics: Recombinant Human CDH3/Cadherin 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp108-Gly654.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CDH3 Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CDH3, His Tag with the EC50 of 1.12ug/ml determined by ELISA (QC Test).,Human CDH3 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: P-Cadherin (CDH3)

Alternative Name: CDH3

Background: Placental-Cadherin (CDH3), a cell adhesion molecule, is associated with the function of cells to bind with other cells and the extracellular matrix (ECM). CDH3 is highly expressed in many malignancies, and has been proved it could be a serum marker to monitor colorectal cancer. Inhibited CDH3 expression could upregulate E-cadherin, downregulated N-cadherin, which may control invasion and migration.

Molecular Weight: 61.11 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: P22223