LI Cadherin Protein (AA 567-777) (hFc Tag)

Catalog No. ABIN7274312

Recombinant LI Cadherin protein expressed in HEK-293 Cells.

Quick Overview for LI Cadherin Protein (AA 567-777) (hFc Tag) (ABIN7274312)

Target: LI Cadherin

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 567-777

Purification tag / Conjugate: This LI Cadherin protein is labelled with hFc Tag.

Purpose: Human CDH17/Cadherin 17 Domain 6-7 Protein

Sequence: Ser567-Gly777

Characteristics: Recombinant Human CDH17/Cadherin 17 Domain 6-7 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ser567-Gly777.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CDH17 Domain 6-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CDH17 Domain 6-7 Antibody, hFc Avi Tag with the EC50 of 0.19ug/ml determined by ELISA.,Human CDH17 Domain 6-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CDH17 Domain 6-7 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris, 150 mM NaCl ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LI Cadherin

Alternative Name: CDH17

Background: Liver-intestine cadherin (CDH17) has been known to function as a tumor stimulator and diagnostic marker for almost two decades.In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.

Molecular Weight: 49.29 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

UniProt: Q12864