CD70 Protein (AA 50-193, Trimer) (Biotin,His tag)

Catalog No. ABIN7274323

This Recombinant CD70 protein is expressed in HEK-293 Cells.

Quick Overview for CD70 Protein (AA 50-193, Trimer) (Biotin,His tag) (ABIN7274323)

Target: CD70 (CD70 Molecule (CD70))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 50-193, Trimer

Purification tag / Conjugate: This CD70 protein is labelled with Biotin,His tag.

Purpose: Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling)

Sequence: Leu50-Pro193

Characteristics: Recombinant Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Leu50-Pro193 trimer design.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer), His Tag with the EC50 of 12.1ng/ml determined by ELISA (QC Test).,Biotinylated Human CD27 Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human CD27 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD70 (CD70 Molecule (CD70))

Alternative Name: CD27 Ligand

Background: CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.  CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Molecular Weight: 51.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

UniProt: P32970

Pathways: Cancer Immune Checkpoints