Adipsin Protein (AA 21-253) (His tag)

Catalog No. ABIN7274333

Recombinant Adipsin protein expressed in HEK-293 Cells.

Quick Overview for Adipsin Protein (AA 21-253) (His tag) (ABIN7274333)

Target: Adipsin (CFD) (Complement Factor D (CFD))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rhesus Monkey

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-253

Purification tag / Conjugate: This Adipsin protein is labelled with His tag.

Purpose: Rhesus macaque Complement Factor D/CFD Protein

Sequence: Gln21-Ala253

Characteristics: Recombinant Rhesus macaque Complement Factor D/CFD Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Ala253.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Rhesus macaque Complement Factor D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA.,Rhesus macaque Complement Factor D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Rhesus macaque Complement Factor D is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris, 150 mM NaCl ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Adipsin (CFD) (Complement Factor D (CFD))

Alternative Name: Complement Factor D

Background: Complement factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium.

Molecular Weight: 25.7 kDa. Due to glycosylation, the protein migrates to 26-30 kDa based on Bis-Tris PAGE result.

UniProt: H9EXC1

Pathways: Complement System