Adipsin Protein (AA 26-259) (hFc Tag)

Catalog No. ABIN7274336

Recombinant Adipsin protein expressed in HEK-293 Cells.

Quick Overview for Adipsin Protein (AA 26-259) (hFc Tag) (ABIN7274336)

Target: Adipsin (CFD) (Complement Factor D (CFD))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-259

Purification tag / Conjugate: This Adipsin protein is labelled with hFc Tag.

Purpose: Mouse Complement Factor D/CFD Protein

Sequence: Ile26-Ser259

Characteristics: Recombinant Mouse Complement Factor D/CFD Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ile26-Ser259.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse Complement Factor D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse Complement Factor D is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 500 mM NaCl ( pH 7.4). Normally 8 % mannitol is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3 months after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Adipsin (CFD) (Complement Factor D (CFD))

Alternative Name: Complement Factor D

Background: Complement factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium.

Molecular Weight: 52.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

UniProt: P03953

Pathways: Complement System