CLEC1A Protein (AA 74-280) (Fc Tag)
Quick Overview for CLEC1A Protein (AA 74-280) (Fc Tag) (ABIN7274352)
Target
See all CLEC1A ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 74-280
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Purification tag / Conjugate
- This CLEC1A protein is labelled with Fc Tag.
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Purpose
- Human CLEC-1 Protein
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Sequence
- Gln74-Asp280
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Characteristics
- Recombinant Human CLEC-1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Gln74-Asp280.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human CLEC-1, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC-1 Antibody, hFc Tag with the EC50 22.1ng/ml determined by ELISA. See testing image for detail.
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C-Type Lectin Domain Family 1, Member A (CLEC1A) (AA 1-280) protein (His tag)
custom-made CLEC1A Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
C-Type Lectin Domain Family 1, Member A (CLEC1A) (AA 1-269) protein (His tag)custom-made CLEC1A Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
C-Type Lectin Domain Family 1, Member A (CLEC1A) (AA 73-269) protein (Fc Tag)CLEC1A Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
C-Type Lectin Domain Family 1, Member A (CLEC1A) (AA 1-280) protein (GST tag)CLEC1A Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
C-Type Lectin Domain Family 1, Member A (CLEC1A) protein (Myc-DYKDDDDK Tag)CLEC1A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CLEC1A (C-Type Lectin Domain Family 1, Member A (CLEC1A))
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Alternative Name
- CLEC-1
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Background
- C-type lectin-like receptor-1 (CLEC-1) is a member of the Dectin-1 cluster of pattern recognition receptors (PRRs). It is involved in host immunity, has immunoregulatory function, and supports allograft tolerance. CLEC-1 may act as a negative regulator of Dectin-1 induced host inflammatory response via suppressing neutrophils recruitment and production of pro-inflammatory cytokine IL-1β production in response to A. fumigatus keratitis.
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Molecular Weight
- 51.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
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UniProt
- Q8NC01
Target
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