SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) (S144A) Protein

Catalog No. ABIN7274373

Product Details

Target: SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)

Protein Type: Recombinant

Protein Characteristics: S144A

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purpose: SARS-CoV-2 3CLpro/3C-like Protease Protein (S144A)

Sequence: Ser1-Gln306 (S144A)

Characteristics: Recombinant SARS-CoV-2 3CLpro/3C-like Protease Protein (S144A) is expressed from E.coli without tag.It contains Ser1-Gln306(S144A).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 25 mM HEPES, 2.5 mM DTT, 10 % Glycerol ( pH 7.5).

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)

Alternative Name: 3CLpro (SARS-CoV NSP8 Products)

Synonyms: pp1a Protein, pp1ab Protein, orf1ab polyprotein (pp1ab) Protein, orf1ab Protein

Target Type: Viral Protein

Background: 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase.

Molecular Weight: 33.78 kDa same as Tris-Bis PAGE result.

NCBI Accession: YP_009725301