SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) (AA 1-306), (E166A) (Active) Protein

Catalog No. ABIN7274379

This Recombinant SARS-Coronavirus Nonstructural Protein 8 protein is produced in Escherichia coli (E. coli).

Quick Overview for SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) (AA 1-306), (E166A) (Active) Protein (ABIN7274379)

Target: SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 1-306, E166A

Purpose: SARS-CoV-2 3CLpro/3C-like Protease Protein (E166A)

Sequence: Ser1-Gln306(E166A)

Characteristics: Recombinant SARS-CoV-2 3CLpro/3C-like Protease Protein (E166A) is expressed from E.coli without tag.It contains Ser1-Gln306(E166A).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: SARS-CoV-2 3CLpro (E166A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SARS-CoV-2 3CLpro (E166A) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in PBS ( pH 7.4).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)

Alternative Name: 3CLpro

Target Type: Viral Protein

Background: 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase.

Molecular Weight: 34.4kDa same as Bis-Tris PAGE result.

NCBI Accession: YP_009725301