CSF1R Protein (AA 20-512) (Fc Tag)

Catalog No. ABIN7274424

This Recombinant CSF1R protein is produced in HEK-293 Cells.

Quick Overview for CSF1R Protein (AA 20-512) (Fc Tag) (ABIN7274424)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-512

Purification tag / Conjugate: This CSF1R protein is labelled with Fc Tag.

Purpose: Human M-CSF R/CSF1R/CD115 Protein

Sequence: Ile20-Glu512

Characteristics: Recombinant Human M-CSF R/CSF1R/CD115 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile20-Glu512.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human M-CSF at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 12.4ng/ml determined by ELISA. The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: M-CSF R

Background: CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

Molecular Weight: 81.3 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Tris-Bis PAGE result.

UniProt: P07333-1

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization