CX3CL1 Protein (AA 25-336) (Biotin,His-Avi Tag)

Catalog No. ABIN7274441

Recombinant CX3CL1 protein expressed in HEK-293 Cells.

Quick Overview for CX3CL1 Protein (AA 25-336) (Biotin,His-Avi Tag) (ABIN7274441)

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-336

Purification tag / Conjugate: This CX3CL1 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Mouse CX3CL1/Fractalkine Protein

Sequence: Gln25-Thr336

Characteristics: Recombinant Biotinylated Mouse CX3CL1/Fractalkine Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln25-Thr336.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Biotinylated Mouse CX3CL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Mouse CX3CL1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Alternative Name: CX3CL1

Background: Fractalkine/CX3C chemokine ligand 1 (CX3CL1) is a chemokine involved in the anticancer function of lymphocytes-mainly NK cells, T cells and dendritic cells. Its increased levels in tumors improve the prognosis for cancer patients, although it is also associated with a poorer prognosis in some types of cancers, such as pancreatic ductal adenocarcinoma.

Molecular Weight: 36 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Bis-Tris PAGE result.

UniProt: O35188

Pathways: Synaptic Membrane