CXCL13 Protein (AA 22-109) (His tag)
Quick Overview for CXCL13 Protein (AA 22-109) (His tag) (ABIN7274449)
Target
See all CXCL13 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 22-109
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Purification tag / Conjugate
- This CXCL13 protein is labelled with His tag.
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Purpose
- Mouse CXCL13/BCA-1 Protein
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Sequence
- Ile22-Ala109
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Characteristics
- Recombinant Mouse CXCL13/BCA-1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ile22-Ala109.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse CXCL13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 1-109) protein (GST tag)
CXCL13 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 23-109) protein (His-SUMO Tag)CXCL13 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 23-109) (Active) protein (hFc Tag)CXCL13 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 22-109) (Active) protein (hFc Tag)CXCL13 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 23-109) protein (GST tag)CXCL13 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 99-330) protein (Fc Tag)CXCL13 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ADA, AM, IA, ScA
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 23-109) protein (His tag)CXCL13 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 500 mM NaCl ( pH 7.5). Normally 8 % mannitol is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CXCL13 (Chemokine (C-X-C Motif) Ligand 13 (CXCL13))
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Alternative Name
- CXCL13
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Background
- Recent studies have implicated chemokines in microglial activation and pathogenesis of neuropathic pain. C-X-C motif chemokine 13 (CXCL13) is a B lymphocyte chemoattractant that activates CXCR5. Using the spinal nerve ligation (SNL) model of neuropathic pain, CXCL13 was persistently upregulated in spinal cord neurons after SNL, resulting in spinal astrocyte activation via CXCR5 in mice.
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Molecular Weight
- 11.7 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Bis-Tris PAGE result.
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UniProt
- O55038
Target
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