CD226 Protein (CD226) (AA 19-247) (Fc Tag)
Quick Overview for CD226 Protein (CD226) (AA 19-247) (Fc Tag) (ABIN7274457)
Target
See all CD226 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 19-247
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Purification tag / Conjugate
- This CD226 protein is labelled with Fc Tag.
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Purpose
- Human DNAM-1/CD226 Protein
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Sequence
- Glu19-Asn247
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Characteristics
- Recombinant Human DNAM-1/CD226 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu19-Asn247.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human CD155, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 4.8μg/ml determined by ELISA. The affinity constant of 0.234 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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CD226 (CD226) (AA 19-247) protein (His-Avi Tag)
CD226 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
CD226 (CD226) (AA 19-252) protein (His tag)CD226 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD226 (CD226) (AA 19-247) protein (His-Avi Tag,Biotin)CD226 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
CD226 (CD226) (AA 19-247) protein (mFc-His Tag)CD226 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD226 (CD226) (AA 19-252) (Active) protein (His tag)CD226 Origin: Cynomolgus Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD226 (CD226) protein (His tag)CD226 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
CD226 (CD226) (AA 19-254) protein (Fc Tag)CD226 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD226 (CD226) proteinCD226 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
CD226 (CD226) (AA 19-247) protein (hIgG-His-tag)CD226 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
CD226 (CD226) (Active) protein (His tag)CD226 Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD226
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Alternative Name
- DNAM-1
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Background
- DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
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Molecular Weight
- 52.8 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.
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UniProt
- Q15762
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Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
Target
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