CD226 Protein (CD226) (His tag)

Catalog No. ABIN7320356

This Recombinant CD226 protein is expressed in HEK-293 Cells.

Quick Overview for CD226 Protein (CD226) (His tag) (ABIN7320356)

Target: CD226

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: ELISA

Purity: > 97 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD226 protein is labelled with His tag.

Purpose: Recombinant Mouse CD226/DNAM-1 Protein (His Tag)

Sequence: Met 1-Pro 254

Characteristics: A DNA sequence encoding the mouse DNAM1 (NP_848802.2) extracellular domain (Met 1-Pro 254) was fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized mouse DNAM1 at 2 μg/ml (100 μl/well) can bind mouse CD155 with a linear ranger of 6. 4-160 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD226

Alternative Name: CD226/DNAM-1

Background: BC051526,CD226,CD226 antigen,CD226 Molecule,DNAM-1,DNAM1,DNAM1 adhesion glycoprotein,DNAX accessory molecule 1,DNAX accessory molecule-1,PTA1,Pta1,T lineage-specific activation antigen 1 antigen,TLiSA1,platelet and T cell activation antigen 1,The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD226, also known as PTA1 or DNAM-1, is a member of the immunoglobulin superfamily containing 2 Ig-like domains of the V-set. High rate of CD226 (Cluster of Differentiation 226) is found on the surface of natural killer cells, platelets, monocytes and a subset of T cells. CD226 have binding sites with CD112 and CD155 and mediate cellular adhesion to other cells containing its ligands.

Molecular Weight: 28.2 kDa

NCBI Accession: NP_848802

UniProt: Q5DW69

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints