DKK3 Protein (AA 23-350) (Biotin,His-Avi Tag)
Quick Overview for DKK3 Protein (AA 23-350) (Biotin,His-Avi Tag) (ABIN7274471)
Target
Protein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 23-350
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Purification tag / Conjugate
- This DKK3 protein is labelled with Biotin,His-Avi Tag.
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Purpose
- Biotinylated Human DKK3 Protein
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Sequence
- Pro23-Ile350
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Characteristics
- Recombinant Biotinylated Human DKK3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro23-Ile350.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Biotinylated Human DKK3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Biotinylated Human DKK3 is greater than 95% as determined by SEC-HPLC.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- DKK3
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Background
- Dkk-3, also known as REIC (Reduced Expansion in Immortalized Cells), is one of four numbered members of the Dickkopf family of Wnt antagonists.DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
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Molecular Weight
- 39.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
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UniProt
- Q9UBP4
Target
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