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DKK3 Protein (AA 23-350) (Biotin,His-Avi Tag)

Recombinant DKK3 protein expressed in HEK-293 Cells.
Catalog No. ABIN7274471
$715.42
Plus shipping costs $50.00
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Quick Overview for DKK3 Protein (AA 23-350) (Biotin,His-Avi Tag) (ABIN7274471)

Target

DKK3

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 16
  • 4
  • 1
  • 1
Human

Source

  • 9
  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
HEK-293 Cells

Purity

> 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC
  • Protein Characteristics

    AA 23-350

    Purification tag / Conjugate

    This DKK3 protein is labelled with Biotin,His-Avi Tag.

    Purpose

    Biotinylated Human DKK3 Protein

    Sequence

    Pro23-Ile350

    Characteristics

    Recombinant Biotinylated Human DKK3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro23-Ile350.

    Sterility

    0.22 μm filtered

    Endotoxin Level

    Less than 1 EU per μg by the LAL method.

    Biological Activity Comment

    Biotinylated Human DKK3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Biotinylated Human DKK3 is greater than 95% as determined by SEC-HPLC.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

    Buffer

    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

    Storage

    -20 °C,-80 °C

    Storage Comment

    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    DKK3

    Background

    Dkk-3, also known as REIC (Reduced Expansion in Immortalized Cells), is one of four numbered members of the Dickkopf family of Wnt antagonists.DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

    Molecular Weight

    39.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

    UniProt

    Q9UBP4
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