DKK1 Protein (AA 178-266, C-Term) (hFc-Avi Tag,Biotin)

Catalog No. ABIN7274475

This Recombinant DKK1 protein is produced in HEK-293 Cells.

Quick Overview for DKK1 Protein (AA 178-266, C-Term) (hFc-Avi Tag,Biotin) (ABIN7274475)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 178-266, C-Term

Purification tag / Conjugate: This DKK1 protein is labelled with hFc-Avi Tag,Biotin.

Purpose: Biotinylated Human DKK1 C terminal Domain Protein

Sequence: Met178-His266

Characteristics: Recombinant Biotinylated Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Met178-His266.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test).,Immobilized Anti-DKK1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA (QC Test).,Biotinylated Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 20 mM NaAc, 150 mM NaCl ( pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM NaAc, 150 mM NaCl ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK1

Background: Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.

Molecular Weight: 38.69 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.

UniProt: O94907

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation