DLK1 Protein (AA 24-303) (hFc Tag)

Catalog No. ABIN7274478

This Recombinant DLK1 protein is produced in HEK-293 Cells.

Quick Overview for DLK1 Protein (AA 24-303) (hFc Tag) (ABIN7274478)

Target: DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-303

Purification tag / Conjugate: This DLK1 protein is labelled with hFc Tag.

Purpose: Human DLK1 Protein

Sequence: Ala24-Gln303

Characteristics: Recombinant Human DLK1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala24-Gln303.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human DLK1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-DLK1 Antibody, hFc Avi Tag with the EC50 of 39.2ng/ml determined by ELISA.,Human DLK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human DLK1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))

Alternative Name: DLK1

Background: Paternally inherited genetic defects of DLK1 were identified in four families with nonsyndromic CPP and a metabolic phenotype. DLK1 encodes a transmembrane protein that is important for adipose tissue homeostasis and neurogenesis and is located in the imprinted chromosome 14q32 region associated with Temple syndrome.

Molecular Weight: 56.58 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: P80370