Desmoglein 2 Protein (DSG2) (AA 50-609) (His tag)

Catalog No. ABIN7274500

This Recombinant Desmoglein 2 protein is expressed in HEK-293 Cells.

Quick Overview for Desmoglein 2 Protein (DSG2) (AA 50-609) (His tag) (ABIN7274500)

Target: Desmoglein 2 (DSG2)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 50-609

Purification tag / Conjugate: This Desmoglein 2 protein is labelled with His tag.

Purpose: Human DSG-2/Desmoglein-2 Protein

Sequence: Ala50-Gly609

Characteristics: Recombinant Human DSG-2/Desmoglein-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala50-Gly609.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human DSG-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DSG-2 Antibody, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Desmoglein 2 (DSG2)

Alternative Name: DSG-2

Background: Desmoglein-2 (DSG-2) as the primary high-affinity receptor used by adenoviruses Ad3, Ad7, Ad11 and Ad14. These serotypes represent key human pathogens causing respiratory and urinary tract infections. In epithelial cells, adenovirus binding of DSG-2 triggers events reminiscent of epithelial-to-mesenchymal transition, leading to transient opening of intercellular junctions.

Molecular Weight: 63.6 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.

UniProt: Q14126

Pathways: Cell-Cell Junction Organization