Desmoglein 3 Protein (DSG3) (AA 50-615) (His tag)
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- Target See all Desmoglein 3 (DSG3) Proteins
- Desmoglein 3 (DSG3)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 50-615
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Desmoglein 3 protein is labelled with His tag.
- Purpose
- Human DSG3 Protein
- Sequence
- Glu50-Arg615
- Characteristics
- Recombinant Human DSG3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu50-Arg615.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human DSG3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-DSG3 Antibody, hFc Tag with the EC50 8.5ng/ml determined by ELISA. See testing image for detail.
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Desmoglein 3 (DSG3) (AA 50-617) protein (His tag)
DSG3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Desmoglein 3 (DSG3) (AA 50-615) protein (His tag)Crystallography grade DSG3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Desmoglein 3 (DSG3) (AA 50-615) protein (His-SUMO Tag)DSG3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Desmoglein 3 (DSG3)
- Alternative Name
- DSG3 (DSG3 Products)
- Synonyms
- DSG3 Protein, CDHF6 Protein, PVA Protein, bal Protein, balding Protein, desmoglein 3 Protein, DSG3 Protein, Dsg3 Protein
- Background
- To identify genes that could potentially serve as molecular therapeutic markers for human head and neck cancer (HNC),DSG3 is identified overexpressed in HNC, with the degree of overexpression associated with clinicopathologic features of the tumor. Inhibition of DSG3 significantly suppresses carcinogenic potential in cellular and in vivo animal studies. DSG3 is a potential molecular target in the development of adjuvant therapy for HNC.
- Molecular Weight
- 64 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.
- UniProt
- P32926
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