ECSCR Protein (AA 25-124) (hFc Tag)

Catalog No. ABIN7274507

Recombinant ECSCR protein expressed in HEK-293 Cells.

Quick Overview for ECSCR Protein (AA 25-124) (hFc Tag) (ABIN7274507)

Target: ECSCR (Endothelial Cell Surface Expressed Chemotaxis and Apoptosis Regulator (ECSCR))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-124

Purification tag / Conjugate: This ECSCR protein is labelled with hFc Tag.

Purpose: Human ECSCR Protein

Sequence: Gln25-Ala124

Characteristics: Recombinant Human ECSCR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln25-Ala124.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human ECSCR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human ECSCR is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ECSCR (Endothelial Cell Surface Expressed Chemotaxis and Apoptosis Regulator (ECSCR))

Alternative Name: ECSCR

Background: Endothelial cell-specific chemotaxis receptor (ECSCR) is a cell surface protein expressed by blood endothelial cells with roles in endothelial cell migration and signal transduction. Zebrafish ecscr is expressed in angioblasts and in axial vessels during angioblast migration and vasculogenesis. Morpholino-directed ecscr knockdown resulted in defective angioblast migration in the posterior lateral plate mesoderm, a process known to depend on vascular endothelial-derived growth factor (VEGF).

Molecular Weight: 36.8 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: Q19T08