Endoglin Protein (ENG) (AA 26-586) (His-Avi Tag)

Catalog No. ABIN7274511

This Recombinant Endoglin protein is produced in HEK-293 Cells.

Quick Overview for Endoglin Protein (ENG) (AA 26-586) (His-Avi Tag) (ABIN7274511)

Target: Endoglin (ENG)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-586

Purification tag / Conjugate: This Endoglin protein is labelled with His-Avi Tag.

Purpose: Human Endoglin/CD105 Protein

Sequence: Glu26-Gly586

Characteristics: Recombinant Human Endoglin/CD105 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu26-Gly586.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Endoglin, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Endoglin (ENG)

Alternative Name: Endoglin

Background: Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin is a vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis. Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells. Required for normal extraembryonic angiogenesis and for embryonic heart development. May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis.

Molecular Weight: 63.7 kDa. Due to glycosylation, the protein migrates to 70-82 kDa based on Tris-Bis PAGE result.

UniProt: P17813-1