EGFR Protein (AA 25-645) (Fc Tag)

Catalog No. ABIN7274524

Product Details

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Protein Type: Recombinant

Protein Characteristics: AA 25-645

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This EGFR protein is labelled with Fc Tag.

Purpose: Human EGFR/HER1 Protein

Sequence: Leu25-Ser645

Characteristics: Recombinant Human EGFR/HER1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu25-Ser645.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human EGF at 2μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 81.0ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR (EGFR Products)

Background: The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

Molecular Weight: 95.2 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins