EGFR Protein (AA 25-645) (His-Avi Tag)

Catalog No. ABIN7274526

This Recombinant EGFR protein is produced in HEK-293 Cells.

Quick Overview for EGFR Protein (AA 25-645) (His-Avi Tag) (ABIN7274526)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-645

Purification tag / Conjugate: This EGFR protein is labelled with His-Avi Tag.

Purpose: Human EGFR/HER1 Protein

Sequence: Leu25-Ser645

Characteristics: Recombinant Human EGFR/HER1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser645.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.05 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

Molecular Weight: 71.5 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result.

UniProt: P00533-1

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins