EGFR Protein (AA 25-378) (His tag,AVI tag,Biotin)

Catalog No. ABIN4949022

This Recombinant EGFR protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for EGFR Protein (AA 25-378) (His tag,AVI tag,Biotin) (ABIN4949022)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Functional Studies (Func)

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 25-378

Purification tag / Conjugate: This EGFR protein is labelled with His tag,AVI tag,Biotin.

Brand: MABSol® - PrecisionAvi

Sequence: AA 25-378

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 41.3 kDa. As a result of glycosylation, the protein migrates as 60-70 kDa under reducing (R) condition, and 60-70 kDa under non-reducing (NR) condition (SDS-PAGE).
This product is biotinylated with precision by using the Avitag™ technology, resulting in precise, single residue biotinylation at the lysine of the tag. This leads to minimal interence with protein binding, structure, and function, as well as uniform orientation upon immobilization.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

References

Mortensen, Skovsgaard, Okholm, Scavenius, Dupont, Rosen, Enghild, Kjems, Gothelf: "Small-Molecule Probes for Affinity-Guided Introduction of Biocompatible Handles on Metal-Binding Proteins." in: Bioconjugate chemistry, Vol. 29, Issue 9, pp. 3016-3025, (2019) (PubMed).

Target Details

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGF R

Background: The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. The type III EGF deletion-mutant receptor (EGFRvIII) is the most common mutation and was first identified in primary human glioblastoma tumors, EGFR gene amplification is correlated with the structural rearrangement of the gene. The EGFRvIII gene has an in-frame deletion of 801 base pairs, corresponding to exons 2-7 in the mRNA, resulting in the deletion of amino acids 30-297 in the extracellular domain and the generation of a glycine at the fusion point.

Molecular Weight: 42.2 kDa

NCBI Accession: NP_001333870

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins