ENPP2 Protein (AA 49-862) (His tag)
Quick Overview for ENPP2 Protein (AA 49-862) (His tag) (ABIN7274543)
Target
See all ENPP2 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 49-862
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Purification tag / Conjugate
- This ENPP2 protein is labelled with His tag.
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Purpose
- Mouse ENPP-2/Autotaxin Protein
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Sequence
- Ser49-Ile862
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Characteristics
- Recombinant Mouse ENPP-2/Autotaxin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser49-Ile862.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse ENPP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is > 10000 pmol/min/ug.,The purity of Mouse ENPP-2 is greater than 95% as determined by SEC-HPLC.
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Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2) protein (His tag)
ENPP2 Origin: Mouse Host: HEK-293 Cells Recombinant > 93 % as determined by SDS-PAGE
Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2) (AA 197-338) protein (His-IF2DI Tag)ENPP2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2) (AA 49-863) protein (His tag)ENPP2 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- ENPP2 (Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2))
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Alternative Name
- ENPP-2
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Background
- Ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2) also known as Autotaxin, is a secreted lysophospholipase D, which hydrolyzes lysophosphatidylcholine (LPC) into Lysophosphatidic acid (LPA). ENPP2 is an essential protein for normal development and its altered expression is associated with various human diseases.
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Molecular Weight
- 94.7 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Bis-Tris PAGE result.
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UniProt
- Q9R1E6
Target
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