EPH Receptor A4 Protein (EPHA4) (AA 20-547) (His tag)
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- Target See all EPH Receptor A4 (EPHA4) Proteins
- EPH Receptor A4 (EPHA4)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-547
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This EPH Receptor A4 protein is labelled with His tag.
- Purpose
- Human EPHA4 Protein
- Sequence
- Val20-Thr547
- Characteristics
- Recombinant Human EPHA4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val20-Thr547.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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EPH Receptor A4 (EPHA4) (AA 20-547) protein (His tag)
EPHA4 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
EPH Receptor A4 (EPHA4) (AA 887-986) protein (GST tag)EPHA4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
EPH Receptor A4 (EPHA4) (AA 1-105) protein (GST tag)EPHA4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
EPH Receptor A4 (EPHA4) (AA 1-986) protein (GST tag)EPHA4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
EPH Receptor A4 (EPHA4) (AA 570-986) (Active) protein (GST tag,His tag)EPHA4 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 99 % as determined by reducing SDS-PAGE. Active
EPH Receptor A4 (EPHA4) (Active) protein (His tag)EPHA4 Origin: Rat Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
EPH Receptor A4 (EPHA4) (AA 20-547) protein (His tag)EPHA4 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
EPH Receptor A4 (EPHA4) (Active) protein (Fc Tag)EPHA4 Origin: Rat Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
EPH Receptor A4 (EPHA4) (AA 20-547) (Active) protein (His tag)EPHA4 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- EPH Receptor A4 (EPHA4)
- Alternative Name
- EPHA4 (EPHA4 Products)
- Background
- The expression and activation of EphA4 in the various cell types in a knee joint was upregulated upon an intraarticular injury. To determine if EphA4 signaling plays a role in osteoarthritis, we determined whether deficient EphA4 expression (in EphA4 knockout mice) or upregulation of the EphA4 signaling (with the EfnA4-fc treatment) would alter cellular functions of synoviocytes and articular chondrocytes. In synoviocytes, deficient EphA4 expression enhanced, whereas activation of the EphA4 signaling reduced, expression and secretion of key inflammatory cytokines and matrix metalloproteases.
- Molecular Weight
- 59.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P54764-1
- Pathways
- RTK Signaling
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