FcRH5 (AA 16-851) (Active) protein (Biotin,His-Avi Tag)

Catalog No. ABIN7274597

Recombinant FcRH5 protein expressed in HEK-293 Cells.

Quick Overview for FcRH5 (AA 16-851) (Active) protein (Biotin,His-Avi Tag) (ABIN7274597)

Target: FcRH5

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 16-851

Purification tag / Conjugate: Biotin,His-Avi Tag

Purpose: Biotinylated Human FcRH5/FcRL5 Protein

Sequence: Gln16-Gly851

Characteristics: Recombinant Biotinylated Human FcRH5/FcRL5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln16-Gly851.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human FcRH5, His Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FcRH5 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA.,Biotinylated Human FcRH5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-FcRH5 Ab., hFc Tag on ProA-Biosensor can bind Biotinylated Human FcRH5, His Tag with an affinity constant of 13.70 nM as determined in BLI assay (Gator® Prime).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FcRH5

Background: FcRH5 is a cell surface marker enriched on malignant plasma cells when compared to other hematologic malignancies and normal tissues. DFRF4539A is an anti-FcRH5 antibody-drug conjugated to monomethyl auristatin E (MMAE), a potent anti-mitotic agent.

Molecular Weight: 94.19 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.

UniProt: Q96RD9