Fc epsilon RI/FCER1A Protein (AA 24-204) (hFc Tag)

Catalog No. ABIN7274609

Recombinant Fc epsilon RI/FCER1A protein expressed in HEK-293 Cells.

Quick Overview for Fc epsilon RI/FCER1A Protein (AA 24-204) (hFc Tag) (ABIN7274609)

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-204

Purification tag / Conjugate: This Fc epsilon RI/FCER1A protein is labelled with hFc Tag.

Purpose: Mouse Fc epsilon RI alpha/FCER1a Protein

Sequence: Ala24-Gln204

Characteristics: Recombinant Mouse Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala24-Gln204.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse Fc epsilon RI alpha/FCER1a on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse Fc epsilon RI alpha/FCER1a is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Alternative Name: Fc Epsilon RI alpha

Background: Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.

Molecular Weight: 47.7 kDa. Due to glycosylation, the protein migrates to 68-72 kDa based on Bis-Tris PAGE result.

UniProt: P20489

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process