FGFR1 beta (AA 158-355) (Active) protein (His-Avi Tag)

Catalog No. ABIN7274621

Recombinant FGFR1 beta protein expressed in HEK-293 Cells.

Quick Overview for FGFR1 beta (AA 158-355) (Active) protein (His-Avi Tag) (ABIN7274621)

Target: FGFR1 beta

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 158-355

Purification tag / Conjugate: His-Avi Tag

Purpose: Non-biotinylated Human FGFR1 beta (IIIc) Protein

Sequence: Lys158-Thr355

Characteristics: Recombinant Human FGFR1 beta (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys158-Thr355.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-FGFR1 Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human FGFR1 beta (IIIc), His Tag with the EC50 of 0.52ug/ml determined by ELISA (QC Test).,Human FGFR1 beta (IIIc) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGFR1 beta

Background: Fibroblast growth factor receptor 1 (FGFR1) transmits signals through the plasma membrane regulating essential cellular processes like division, motility, metabolism, and death. Overexpression of FGFR1 is observed in numerous tumors and thus constitutes an attractive molecular target for selective cancer treatment.

Molecular Weight: 25.23 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.

UniProt: P11362