Fibroblast Growth Factor Receptor 3 (Achondroplasia, Thanatophoric Dwarfism) (FGFR3) (AA 23-375) (Active) protein (His-Avi Tag)

Catalog No. ABIN7274624

Recombinant Fibroblast Growth Factor Receptor 3 (Achondroplasia, Thanatophoric Dwarfism) protein expressed in HEK-293 Cells.

Quick Overview for Fibroblast Growth Factor Receptor 3 (Achondroplasia, Thanatophoric Dwarfism) (FGFR3) (AA 23-375) (Active) protein (His-Avi Tag) (ABIN7274624)

Target: Fibroblast Growth Factor Receptor 3 (Achondroplasia, Thanatophoric Dwarfism) (FGFR3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-375

Purification tag / Conjugate: His-Avi Tag

Purpose: Non-biotinylated Human FGFR3 alpha (IIIc) Protein

Sequence: Glu23-Gly375

Characteristics: Recombinant Human FGFR3 alpha (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu23-Gly375.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FGFR3 alpha (IIIc) at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test).,Human FGFR3 alpha (IIIc) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Human FGFR3 alpha (IIIc) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Fibroblast Growth Factor Receptor 3 (Achondroplasia, Thanatophoric Dwarfism) (FGFR3)

Alternative Name: FGFR3 alpha

Background: Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain.FGFR3 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts.

Molecular Weight: 41.1 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

UniProt: P22607