FGFR3 beta (IIIb) (AA 127-377) (Active) protein (Biotin,His-Avi Tag)
This Recombinant FGFR3 beta (IIIb) protein is produced in HEK-293 Cells.
Catalog No. ABIN7274628
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Quick Overview for FGFR3 beta (IIIb) (AA 127-377) (Active) protein (Biotin,His-Avi Tag) (ABIN7274628)
Target
FGFR3 beta (IIIb)
Protein Type
Recombinant
Biological Activity
Active
Origin
Human
Source
HEK-293 Cells
Purity
> 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC
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Protein Characteristics
- AA 127-377
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Purification tag / Conjugate
- Biotin,His-Avi Tag
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Purpose
- Biotinylated Human FGFR3 beta (IIIb) Protein
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Sequence
- Asp127-Gly377
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Characteristics
- Recombinant Biotinylated Human FGFR3 beta (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp127-Gly377.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human FGFR3 beta (IIIb) , His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.,Biotinylated Human FGFR3 beta (IIIb) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human FGFR3 beta (IIIb) is greater than 95% as determined by SEC-HPLC.
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FGFR3 beta (IIIb) (AA 127-377) (Active) protein (His-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- FGFR3 beta (IIIb)
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Alternative Name
- FGFR3 bata (IIIb)
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Background
- Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain.FGFR3 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis.
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Molecular Weight
- 30.5 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
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UniProt
- P22607
Target
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