Fc gamma RII (CD32) (AA 17-208) (Active) protein (His tag)

Catalog No. ABIN7274645

Recombinant Fc gamma RII protein expressed in HEK-293 Cells.

Quick Overview for Fc gamma RII (CD32) (AA 17-208) (Active) protein (His tag) (ABIN7274645)

Target: Fc gamma RII (CD32)

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 17-208

Purification tag / Conjugate: His tag

Purpose: Cynomolgus Fc gamma RIII/CD16 Protein

Sequence: Gly17-Gln208

Characteristics: Recombinant Cynomolgus Fc gamma RIII/CD16 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly17-Gln208.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Rituximab captured on CM5 Chip via Protein A can bind Cynomolgus Fc gamma RIII, His Tag with an affinity constant of 0.251 uM as determined in SPR assay (Biacore T200).,Cynomolgus Fc gamma RIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Cynomolgus Fc gamma RIII, His Tag on Anti-His-Biosensor, can bind Rituximab with an affinity constant of 0.14 μM as determined in BLI assay (Gator).,The purity of Cynomolgus Fc gamma RIII is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: Fc gamma RIII

Background: Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R, Fc gamma RII and Fc gamma RIII also utilize residues outside this common set.

Molecular Weight: 23.1 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.

UniProt: Q8SPW2