FGFR2 beta (AA 152-378) (Active) protein (hFc Tag)

Catalog No. ABIN7274649

This Recombinant FGFR2 beta protein is produced in HEK-293 Cells.

Quick Overview for FGFR2 beta (AA 152-378) (Active) protein (hFc Tag) (ABIN7274649)

Target: FGFR2 beta

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 152-378

Purification tag / Conjugate: hFc Tag

Purpose: Human FGFR2 beta (IIIb) Protein

Sequence: Arg152-Glu378

Characteristics: Recombinant Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Arg152-Glu378.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human FGF10, No Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb), hFc Tag with an affinity constant of 8.43 nM as determined in SPR assay (Biacore T200).,Immobilized Human FGF-7, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 0.28 ug/ml determined by ELISA.,Immobilized Human FGF basic, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 33.0ng/ml determined by ELISA.,Immobilized Human FGFR2 beta (IIIb) , hFc Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 19.9 ng/ml determined by ELISA.,Human FGFR2 beta (IIIb) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGFR2 beta

Background: Four distinct genes encoding closely related FGF receptors, FGFR1-4, are known. All four genes for FGFRs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGFR1-3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGFR1 and 2 results in receptors containing all three Ig domains, referred to as the alpha  isoform, or only IgII and IgIII, referred to as the beta isoform.

Molecular Weight: 52.2 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.

UniProt: P21802