FOLR1 Protein (AA 25-233) (hFc Tag)

Catalog No. ABIN7274666

Recombinant FOLR1 protein expressed in HEK-293 Cells.

Quick Overview for FOLR1 Protein (AA 25-233) (hFc Tag) (ABIN7274666)

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-233

Purification tag / Conjugate: This FOLR1 protein is labelled with hFc Tag.

Purpose: Human FOLR1 Protein

Sequence: Arg25-Met233

Characteristics: Recombinant Human FOLR1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Arg25-Met233.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FOLR1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-FOLR1 Antibody, hFc Tag with the EC50 of 29.6ng/ml determined by ELISA.,Human FOLR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human FOLR1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Alternative Name: FOLR1

Background: Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH .

Molecular Weight: 51.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

UniProt: P15328

Pathways: Dicarboxylic Acid Transport