Folate Receptor 4 Protein (AA 20-222) (His tag)

Catalog No. ABIN7274671

This Recombinant Folate Receptor 4 protein is expressed in HEK-293 Cells.

Quick Overview for Folate Receptor 4 Protein (AA 20-222) (His tag) (ABIN7274671)

Target: Folate Receptor 4 (FOLR4) (Folate Receptor 4 (Delta) (FOLR4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-222

Purification tag / Conjugate: This Folate Receptor 4 protein is labelled with His tag.

Purpose: Mouse FOLR4/Juno Protein

Sequence: Gly20-Gly222

Characteristics: Recombinant Mouse FOLR4/Juno Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Gly222.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse FOLR4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse FOLR4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Folate Receptor 4 (FOLR4) (Folate Receptor 4 (Delta) (FOLR4))

Alternative Name: FOLR4

Background: Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno, formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor.

Molecular Weight: 24.7 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.

UniProt: Q9EQF4