TNFSF15 Protein (AA 91-251, Trimer) (His-DYKDDDDK Tag)

Catalog No. ABIN7274680

This Recombinant TNFSF15 protein is expressed in HEK-293 Cells.

Quick Overview for TNFSF15 Protein (AA 91-251, Trimer) (His-DYKDDDDK Tag) (ABIN7274680)

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 91-251, Trimer

Purification tag / Conjugate: This TNFSF15 protein is labelled with His-DYKDDDDK Tag.

Purpose: Human TNFSF15 Trimer Protein

Sequence: Asp91-Leu251

Characteristics: Recombinant Human TNFSF15 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-terminus.It contains Asp91-Leu251 trimer design.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human DR3, hFc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).,Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15 (Trimer) , His Tag with an affinity constant of 3.06 nM as determined in SPR assay (Biacore T200).,Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 2.2ug/ml determined by ELISA.,Immobilized Human TNFSF15 Trimer, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA.,Immobilized Human TNFSF15 (Trimer), His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 38.5ng/ml determined by ELISA.,Human TNFSF15 Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Alternative Name: TNFSF15

Background: TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Molecular Weight: 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.

UniProt: O95150

Pathways: Positive Regulation of Endopeptidase Activity, Autophagy