Factor XI Protein (AA 19-625) (His tag)

Catalog No. ABIN7274693

Recombinant Factor XI protein expressed in HEK-293 Cells.

Quick Overview for Factor XI Protein (AA 19-625) (His tag) (ABIN7274693)

Target: Factor XI (F11) (Coagulation Factor XI (F11))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-625

Purification tag / Conjugate: This Factor XI protein is labelled with His tag.

Purpose: Human Coagulation factor XI Protein (pro form)

Sequence: Glu19-Val625

Characteristics: Recombinant Human Coagulation factor XI Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by Thermolysin for an activated form.It contains Glu19-Val625.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Coagulation factor XI, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor XI Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.,Human Coagulation factor XI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >100 pmoles/min/ug.,The purity of Human Coagulation factor XI is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 25 mM HEPES, 150 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Factor XI (F11) (Coagulation Factor XI (F11))

Alternative Name: Coagulation factor XI

Background: Coagulation factor (F)XI has been described as a component of the early phase of the contact pathway of blood coagulation, acting downstream of factor XII.In contrast to its mild hemostatic activities, FXI is known to play a significant role in thrombosis, as it is a demonstrated independent risk factor for deep vein thrombosis, ischemic stroke, and myocardial infarction. A better understanding of the varied and complex role of FXI in both thrombosis and hemostasis will help to allow better prediction of bleeding risk in FXI-deficient patients and also informing the development of targeted agents to inhibit the thrombotic activities of FXI while preserving hemostasis.

Molecular Weight: 69.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: P03951