GARP & Latent TGF beta 1 Complex (AA 20-628), (Y137H) (Active) protein (His-Avi Tag)

Catalog No. ABIN7274702

Recombinant GARP & Latent TGF beta 1 Complex protein expressed in HEK-293 Cells.

Quick Overview for GARP & Latent TGF beta 1 Complex (AA 20-628), (Y137H) (Active) protein (His-Avi Tag) (ABIN7274702)

Target: GARP & Latent TGF beta 1 Complex

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-628, Y137H

Purification tag / Conjugate: His-Avi Tag

Purpose: Non-biotinylated Human GARP (Y137H) &Latent TGF Beta 1 Complex Protein

Sequence: His20-Leu628(GARP(Y137H)) & Leu30-Ser390(Latent TGF Beta 1)

Specificity: Uni-Prot: Q14392 (GARP (Y137H)), P01137 (Latent TGF beta 1)

Characteristics: Recombinant Human GARP(Y137H)&Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His20-Leu628 (GARP(Y137H)) and Leu30-Ser390 (Latent TGF Beta 1).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GARP (Y137H) &Latent TGF Beta 1 Complex, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GARP&Latent TGF Beta 1 Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA.,Human GARP (Y137H) &Latent TGF Beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human GARP (Y137H) &Latent TGF Beta 1 Complex is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GARP & Latent TGF beta 1 Complex

Background: GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.

Molecular Weight: 70.3 kDa (GARP(Y137H)) and 41.4 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 71-75 kDa (GARP(Y137H)), 13 kDa and 42-45 kDa (Latent TGF Beta 1) based on Bis-Tris PAGE result.

UniProt: Q14392