GEP (AA 18-593) (Active) protein (His tag)

Catalog No. ABIN7274732

Recombinant GEP protein expressed in HEK-293 Cells.

Quick Overview for GEP (AA 18-593) (Active) protein (His tag) (ABIN7274732)

Target: GEP

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 18-593

Purification tag / Conjugate: His tag

Purpose: Human GEP Protein

Sequence: Thr18-Leu593

Characteristics: Recombinant Human GEP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr18-Leu593.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Human GEP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human GEP is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GEP

Background: Haploinsufficiency of progranulin (PGRN) is a leading cause of frontotemporal lobar degeneration (FTLD). Loss of PGRN leads to lysosome dysfunction during aging. TMEM106B, a gene encoding a lysosomal membrane protein, is the main risk factor for FTLD with PGRN haploinsufficiency.Loss of both PGRN and TMEM106B results in an increased accumulation of lysosomal vacuoles in the axon initial segment of motor neurons and enhances the manifestation of FTLD phenotypes with a much earlier onset.

Molecular Weight: 62.9 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Bis-Tris PAGE result.

UniProt: P28799