GFRAL Protein (AA 19-351) (His-Avi Tag)

Catalog No. ABIN7274737

This Recombinant GFRAL protein is expressed in HEK-293 Cells.

Quick Overview for GFRAL Protein (AA 19-351) (His-Avi Tag) (ABIN7274737)

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-351

Purification tag / Conjugate: This GFRAL protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human GFRAL/GFR alpha-like Protein

Sequence: Ser19-Glu351

Characteristics: Recombinant Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser19-Glu351.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GFRAL, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 3.3 ng/ml determined by ELISA.,Human GFRAL on Bis-Tris PAGE under reduced condition.The purity is greater than 95%.,Loaded Human GDF15, hFc Tag on ProA-Biosensor can bind Human GFRAL, His-Avi Tag with an affinity constant of 35.50 nM as determined in BLI assay (Gator® Prime).,The purity of-Human GFRAL is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4).Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Alternative Name: GFRAL

Background: GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Molecular Weight: 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Bis-Tris PAGE result.

UniProt: Q6UXV0