GFRA3 Protein (AA 32-382) (His tag)
Quick Overview for GFRA3 Protein (AA 32-382) (His tag) (ABIN7274742)
Target
See all GFRA3 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 32-382
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Purification tag / Conjugate
- This GFRA3 protein is labelled with His tag.
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Purpose
- Human GFRA3 Protein
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Sequence
- Asp32-Trp382
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Characteristics
- Recombinant Human GFRA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp32-Trp382.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human GFRA3, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-GFRA3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. See testing image for detail.
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GDNF Family Receptor alpha 3 (GFRA3) (AA 29-379) protein (His tag)
GFRA3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha 3 (GFRA3) (AA 32-374) protein (His tag)GFRA3 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
GDNF Family Receptor alpha 3 (GFRA3) (AA 1-400) protein (GST tag)GFRA3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
GDNF Family Receptor alpha 3 (GFRA3) (AA 32-374) protein (His tag)GFRA3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
GDNF Family Receptor alpha 3 (GFRA3) protein (Fc Tag)GFRA3 Origin: Mouse Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE
GDNF Family Receptor alpha 3 (GFRA3) (AA 32-374) protein (hIgG-His-tag)GFRA3 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85% by SDS-PAGE SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))
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Alternative Name
- GFRA3
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Background
- GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia.
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Molecular Weight
- 40.3 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
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UniProt
- O60609-1
Target
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