GIP Protein (AA 22-93) (His tag)

Catalog No. ABIN7274746

This Recombinant GIP protein is expressed in HEK-293 Cells.

Quick Overview for GIP Protein (AA 22-93) (His tag) (ABIN7274746)

Target: GIP (Gastric Inhibitory Polypeptide (GIP))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-93

Purification tag / Conjugate: This GIP protein is labelled with His tag.

Purpose: Human GIP Protein

Sequence: Glu22-Gln93

Characteristics: Recombinant Human GIP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Gln93.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GIP, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GIP Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA.,Human GIP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human GIP is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: GIP (Gastric Inhibitory Polypeptide (GIP))

Alternative Name: GIP

Background: The potential application of glucose-dependent insulinotropic polypeptide (gastric inhibitory polypeptide, GIP) in the management of obesity and type 2 diabetes has been controversial. Initial interest in the therapeutic use of GIP was dampened by evidence that its insulinotropic activity was reduced in type 2 diabetes and by reports that it increased glucagon secretion and adipose deposition in non-diabetic individuals.

Molecular Weight: 9.2 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Bis-Tris PAGE result.

UniProt: P09681

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism