GIP Protein (AA 22-93) (His tag)
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- Target See all GIP Proteins
- GIP (Gastric Inhibitory Polypeptide (GIP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-93
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GIP protein is labelled with His tag.
- Purpose
- Human GIP Protein
- Sequence
- Glu22-Gln93
- Characteristics
- Recombinant Human GIP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Gln93.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GIP, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GIP Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product GIP Protein
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Gastric Inhibitory Polypeptide (GIP) (AA 22-93) protein (Fc Tag)
GIP Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Gastric Inhibitory Polypeptide (GIP) (AA 22-85) protein (Fc Tag)GIP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Gastric Inhibitory Polypeptide (GIP) (AA 22-85) protein (His tag)GIP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Gastric Inhibitory Polypeptide (GIP) (AA 1-153) protein (GST tag)GIP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Gastric Inhibitory Polypeptide (GIP) protein (Myc-DYKDDDDK Tag)GIP Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- GIP (Gastric Inhibitory Polypeptide (GIP))
- Alternative Name
- GIP (GIP Products)
- Synonyms
- GIP Protein, xgip Protein, Gludins Protein, RATGLUDINS Protein, gastric inhibitory polypeptide Protein, gastric inhibitory polypeptide L homeolog Protein, GIP Protein, gip Protein, Gip Protein, gip.L Protein
- Background
- The potential application of glucose-dependent insulinotropic polypeptide (gastric inhibitory polypeptide, GIP) in the management of obesity and type 2 diabetes has been controversial. Initial interest in the therapeutic use of GIP was dampened by evidence that its insulinotropic activity was reduced in type 2 diabetes and by reports that it increased glucagon secretion and adipose deposition in non-diabetic individuals.
- Molecular Weight
- 9.2 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Tris-Bis PAGE result.
- UniProt
- P09681
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism
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