GP6 Protein (AA 21-267) (His tag)

Catalog No. ABIN7274760

This Recombinant GP6 protein is produced in HEK-293 Cells.

Quick Overview for GP6 Protein (AA 21-267) (His tag) (ABIN7274760)

Target: GP6 (Glycoprotein VI (Platelet) (GP6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-267

Purification tag / Conjugate: This GP6 protein is labelled with His tag.

Purpose: Human GPVI Protein

Sequence: Gln21-Lys267

Characteristics: Recombinant Human GPVI Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Lys267.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GPVI, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test).,Human GPVI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human GPVI is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GP6 (Glycoprotein VI (Platelet) (GP6))

Alternative Name: GPVI

Background: The immunoglobulin receptor GPVI (glycoprotein VI) is selectively expressed on megakaryocytes and platelets and is currently recognized as a receptor for not only collagen but also a variety of plasma and vascular proteins, including fibrin, fibrinogen, laminin, fibronectin, and galectin-3.

Molecular Weight: 28 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

UniProt: Q9HCN6