Glypican 3 Protein (GPC3) (AA 25-559) (His-Avi Tag,Biotin)
Quick Overview for Glypican 3 Protein (GPC3) (AA 25-559) (His-Avi Tag,Biotin) (ABIN7274775)
Target
See all Glypican 3 (GPC3) ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 25-559
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Purification tag / Conjugate
- This Glypican 3 protein is labelled with His-Avi Tag,Biotin.
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Purpose
- Biotinylated Human GPC3/Glypican 3 Protein
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Sequence
- Gln25-His559
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Characteristics
- Recombinant Biotinylated Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln25-His559.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Anti-GPC3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPC3, His Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
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Glypican 3 (GPC3) (AA 25-559) protein (His tag)
GPC3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glypican 3 (GPC3) (AA 25-559) protein (His tag)GPC3 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glypican 3 (GPC3) protein (Fc Tag)GPC3 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Glypican 3 (GPC3) (AA 438-554) (Active) protein-VLPGPC3 Origin: Human Host: HEK-293 Cells VLP > 95 % as determined by HPLC Imm, ELISA, Func, SPR Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (Fc Tag)GPC3 Origin: Cynomolgus Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE Func Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (Fc Tag)GPC3 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE Func Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (His tag)GPC3 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (His tag,AVI tag,Biotin)GPC3 Origin: Human Host: HEK-293 Cells Recombinant >85 % as determined by SDS-PAGE. Active
Glypican 3 (GPC3) protein (His tag)GPC3 Origin: Mouse Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Glypican 3 (GPC3) protein (His tag)GPC3 Origin: Rhesus Monkey Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- Glypican 3 (GPC3)
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Alternative Name
- GPC3
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Background
- Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
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Molecular Weight
- 63.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 68-80 kDa based on Tris-Bis PAGE result.
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UniProt
- P51654-1
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Pathways
- Glycosaminoglycan Metabolic Process
Target
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