HGFA Protein (AA 35-653) (His tag)

Catalog No. ABIN7274811

This Recombinant HGFA protein is produced in HEK-293 Cells.

Quick Overview for HGFA Protein (AA 35-653) (His tag) (ABIN7274811)

Target: HGFA (Hepatocyte Growth Factor Activator (HGFA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 35-653

Purification tag / Conjugate: This HGFA protein is labelled with His tag.

Purpose: Mouse HGFA Protein (pro form)

Sequence: Gln35-Ser653

Characteristics: Recombinant Mouse HGFA Protein (pro form) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln35-Ser653.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse HGFA (pro form) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse HGFA (pro form) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris,150 mM NaCl, 2 mM CaCl2 ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HGFA (Hepatocyte Growth Factor Activator (HGFA))

Alternative Name: HGFA

Background: Hepatocyte growth factor activator (HGFA) is a serine protease initially identified as a potent activator of hepatocyte growth factor/scatter factor. Hepatocyte growth factor/scatter factor is known to be critically involved in tissue morphogenesis, regeneration, and tumor progression, via its receptor, MET.In vivo, HGFA also activates macrophage-stimulating protein, which has roles in macrophage recruitment and inflammatory processes, cellular survival and wound healing through its receptor, RON.

Molecular Weight: 67.9 kDa. Due to glycosylation, the protein (pro form) migrates to 80-100 kDa based on Bis-Tris PAGE result.

UniProt: Q9R098