HLA-G Free Heavy Chain (AA 25-305) (Active) protein (His-Avi Tag)

Catalog No. ABIN7274814

This Recombinant HLA-G Free Heavy Chain protein is produced in Escherichia coli (E. coli).

Quick Overview for HLA-G Free Heavy Chain (AA 25-305) (Active) protein (His-Avi Tag) (ABIN7274814)

Target: HLA-G Free Heavy Chain

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 25-305

Purification tag / Conjugate: His-Avi Tag

Purpose: Non-biotinylated Human HLA-G Free Heavy Chain Protein

Sequence: Gly25-Thr305(C66S)

Characteristics: Recombinant Human HLA-G Free Heavy Chain Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(C66S).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human HLA-G Free Heavy Chain, His Tag at 2ug/ml (100ul/Well) on the. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 29.0ng/ml determined by ELISA (QC Test).,Immobilized Human HLA-G Free Heavy Chain, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human KIR2DL4, hFc Tag with the EC50 of 43.4ng/ml determined by ELISA.,Human HLA-G Free heavy chain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 20 mM Tris, 500 mM NaCl, 20 % Glycerol ( pH 8.0).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HLA-G Free Heavy Chain

Background: HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.

Molecular Weight: 35.5 kDa. The protein migrates to 36-40 kDa based on Bis-Tris PAGE result.

UniProt: P17693