Hepsin Protein (HPN) (AA 45-417) (His tag)

Catalog No. ABIN7274824

This Recombinant Hepsin protein is expressed in HEK-293 Cells.

Quick Overview for Hepsin Protein (HPN) (AA 45-417) (His tag) (ABIN7274824)

Target: Hepsin (HPN)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 45-417

Purification tag / Conjugate: This Hepsin protein is labelled with His tag.

Purpose: Human HPN Protein

Sequence: Arg45-Leu417

Characteristics: Recombinant Human HPN Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg45-Leu417.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human HPN, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 22.8ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM NaAc,150 mM NaCl ( pH 5.0).

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM NaAc,150 mM NaCl ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Hepsin (HPN)

Alternative Name: HPN

Background: Home parenteral nutrition (HPN) has been proposed as the treatment of choice in patients suffering from intestinal failure (IF) and has been claimed to improve survival and quality of life either in patients with benign disorders or even in those with malignancies. HPN seems to have beneficial effect but it should be considered with caution by the physicians who should take into account the indications of each patient to receive parenteral nutrition, the underlying disease and prognosis and the access of each patient to home care services.

Molecular Weight: 42 kDa. Due to the protein cleaved into the following two chains, so it migrates to 18-22 kDa and 30 kDa based on Tris-Bis PAGE result.

UniProt: P05981

Pathways: Regulation of Hormone Metabolic Process, Sensory Perception of Sound