Hemopexin Protein (HPX) (AA 24-460) (His tag)

Catalog No. ABIN7274828

Recombinant Hemopexin protein expressed in HEK-293 Cells.

Quick Overview for Hemopexin Protein (HPX) (AA 24-460) (His tag) (ABIN7274828)

Target: Hemopexin (HPX)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-460

Purification tag / Conjugate: This Hemopexin protein is labelled with His tag.

Purpose: Mouse HPX Protein

Sequence: Ser24-Gln460

Characteristics: Recombinant Mouse HPX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser24-Gln460.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM MES, 150 mM NaCl ( pH 6.0).

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM MES, 150 mM NaCl ( pH 6.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Hemopexin (HPX)

Alternative Name: HPX

Background: Hemopexin (HPX) serves as scavenger and transporter of toxic plasma heme to the liver. HPX is formed by two four-bladed beta-propeller domains, resembling two thick disks that lock together at a 90 degrees angle. The heme is bound between the two beta-propeller domains in a pocket formed by the interdomain linker peptide.HPX, acting not only as a heme carrier but also displaying transient heme-based ligand binding and (pseudo-)enzymatic properties, could be considered a 'chronosteric' heme-protein.

Molecular Weight: 50 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.

UniProt: Q91X72

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response