IDH1 Protein (AA 2-414) (His tag)

Catalog No. ABIN7274843

Recombinant IDH1 protein expressed in Escherichia coli (E. coli).

Quick Overview for IDH1 Protein (AA 2-414) (His tag) (ABIN7274843)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 2-414

Purification tag / Conjugate: This IDH1 protein is labelled with His tag.

Purpose: Human IDH1 Protein

Sequence: Ser2-Leu414

Characteristics: Recombinant Human IDH1 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Ser2-Leu414.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IDH1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10000 pmol/min/ug.,The purity of Human IDH1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 50 mM Tris, 200 mM NaCl, 1 mM DTT, 10 % Glycerol ( pH 7.5).

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Alternative Name: IDH1

Background: Isocitrate dehydrogenase (IDH) is a metabolic enzyme that converts isocitrate to α-ketoglutarate (α-KG). Genetic gain-of-function mutations in IDH1 and IDH2 confer a neomorphic activity that allow reduction of α -KG to (R)-2- hydroxyglutarate, the accumulation of which results in the development of cancers like low grade gliomas and leukemia.Isocitrate dehydrogenase 1 (IDH1) is the most frequently mutated metabolic gene in cancer.

Molecular Weight: 47.35 kDa same as Bis-Tris PAGE result.

UniProt: O75874

Pathways: Warburg Effect