IFNA Protein (AA 27-243) (His tag)
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- Target See all IFNA Proteins
- IFNA (Interferon alpha (IFNA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-243
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IFNA protein is labelled with His tag.
- Purpose
- Human IFN alpha/beta R2 Protein
- Sequence
- Ile27-Lys243
- Characteristics
- Recombinant Human IFN alpha/beta R2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile27-Lys243.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 11.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product IFNA Protein
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Interferon alpha (IFNA) protein (His-Avi Tag)
IFNA Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interferon alpha (IFNA) protein (His-Avi Tag,Biotin)IFNA Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interferon alpha (IFNA) (AA 27-429) protein (His-Avi Tag)IFNA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interferon alpha (IFNA) (AA 27-429) protein (Fc Tag)IFNA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IFNA (Interferon alpha (IFNA))
- Alternative Name
- IFN alpha (IFNA Products)
- Background
- Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α's effects on DCs are mediated through miRNAs as well.
- Molecular Weight
- 25.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
- Pathways
- JAK-STAT Signaling, TLR Signaling, Hepatitis C, Inflammasome
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